Human Carbonic anhydrase ELISA Kit | CA elisa kit

Human Carbonic anhydrase ELISA Kit

Cat. Num. AAA723069

• Gene Names: cahz; cah; CAH-Z; cb588; id:ibd5016; wu:fj65c09; wu:cegs2190
• Reactivity: Human
• Synonyms: Carbonic anhydrase; Human Carbonic anhydrase ELISA Kit; CA elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA elisa kit

Principle of the Assay: CA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CA. Standards or samples are then added to the microtiter plate wells and CA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CA present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CA are added to each well to "sandwich" the CA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18858379
• NCBI GeneID: 30331
• NCBI Accession #: NP_571185.1
• NCBI GenBank Nucleotide #: NM_131110.1
• Molecular Weight: 28,677 Da
• NCBI Official Full Name: carbonic anhydrase
• NCBI Official Synonym Full Names: carbonic anhydrase
• NCBI Official Symbol: cahz
• NCBI Official Synonym Symbols: cah; CAH-Z; cb588; id:ibd5016; wu:fj65c09; wu:cegs2190
• NCBI Protein Information: carbonic anhydrase; ca2b; zCAb; zCA2-like b; carbonate dehydratase
• UniProt Protein Name: Carbonic anhydrase
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: cahz
• UniProt Synonym Gene Names: cah-z
• UniProt Entry Name: CAHZ_DANRE

Uniprot Description

Reversible hydration of carbon dioxide.