Human Putative methyltransferase KIAA1456 ELISA Kit | C8orf79 elisa kit

Human Putative methyltransferase KIAA1456 (C8orf79) ELISA Kit

Cat. Num. AAA280281

• Gene Names: TRMT9B; TRM9L; hTRM9L; C8orf79; KIAA1456
• Reactivity: Human
• Synonyms: Putative methyltransferase KIAA1456; Human Putative methyltransferase KIAA1456 (C8orf79) ELISA Kit; C8orf79 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human C8orf79. No significant cross-reactivity or interference between Human C8orf79 and analogues was observed.
• Sequence Length: 454

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for C8orf79 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate C8orf79 in samples. An antibody specific for C8orf79 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC8orf79 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C8orf79 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C8orf79 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 153251913
• NCBI GeneID: 57604
• NCBI Accession #: NP_065895.2
• NCBI GenBank Nucleotide #: NM_020844.2
• UniProt Accession #: Q9P272; Q96AW6
• Molecular Weight: 13,671 Da
• NCBI Official Full Name: probable tRNA methyltransferase 9-like protein isoform 1
• NCBI Official Synonym Full Names: tRNA methyltransferase 9B (putative)
• NCBI Official Symbol: TRMT9B
• NCBI Official Synonym Symbols: TRM9L; hTRM9L; C8orf79; KIAA1456
• NCBI Protein Information: probable tRNA methyltransferase 9-like protein
• UniProt Protein Name: Probable tRNA methyltransferase 9-like protein
• UniProt Gene Name: KIAA1456
• UniProt Synonym Gene Names: C8orf79; TRM9L

Uniprot Description

KIAA1456: Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytoplasm; nucleus

Molecular Function: ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA (uracil) methyltransferase activity; tRNA binding; tRNA methyltransferase activity

Biological Process: tRNA methylation; tRNA modification; tRNA wobble uridine modification