Human Complement C4-A ELISA Kit | C4A elisa kit

Human Complement C4-A ELISA Kit

Cat. Num. AAA763793

• Gene Names: C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
• Reactivity: Human
• Synonyms: Complement C4-A; Human Complement C4-A ELISA Kit; C4A/C4AD/C4S/CO4/CPAMD2/RG/Complement Component 4a; C4A elisa kit

• Reactivity: Human
• Sequence Length: 1744

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C4A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human C4A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human C4A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human C4A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human C4A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 67190748
• NCBI GeneID: 720
• NCBI Accession #: NP_009224.2
• NCBI GenBank Nucleotide #: NM_007293.2
• Molecular Weight: 187,704 Da
• NCBI Official Full Name: complement C4-A isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component 4A (Rodgers blood group)
• NCBI Official Symbol: C4A
• NCBI Official Synonym Symbols: C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
• NCBI Protein Information: complement C4-A
• UniProt Protein Name: Complement C4-A
• Protein Family: Complement C4
• UniProt Gene Name: C4A
• UniProt Synonym Gene Names: CO4; CPAMD2
• UniProt Entry Name: CO4A_HUMAN

NCBI Description

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

C4A: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4A are the cause of complement component 4A deficiency (C4AD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. Defects in C4A are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: complement component C1q binding; endopeptidase inhibitor activity

Biological Process: complement activation; complement activation, classical pathway; inflammatory response; innate immune response; regulation of complement activation

Disease: Blood Group, Chido/rodgers System; Complement Component 4a Deficiency