Rat Complement Fragment 3A ELISA Kit | C3A elisa kit

Rat Complement Fragment 3A ELISA Kit

Cat. Num. AAA44753

• Reactivity: Rat
• Synonyms: Complement Fragment 3A; Rat Complement Fragment 3A ELISA Kit; C3A elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat C3A and analogues was observed.
• Sequence Length: 473

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 15.6ng/ml-500ng/ml.
• Sensitivity: 2.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated C3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat C3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3A and C3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for C3A elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated C3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat C3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3A and C3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14091740
• NCBI GeneID: 84007
• NCBI Accession #: NP_114449.1
• NCBI GenBank Nucleotide #: NM_032060.1
• UniProt Accession #: O55197
• Molecular Weight: 52,896 Da
• NCBI Official Full Name: C3a anaphylatoxin chemotactic receptor
• NCBI Official Synonym Full Names: complement component 3a receptor 1
• NCBI Official Symbol: C3ar1
• NCBI Protein Information: C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R
• UniProt Protein Name: C3a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C3ar1
• UniProt Synonym Gene Names: C3AR; C3a-R
• UniProt Entry Name: C3AR_RAT

NCBI Description

receptor for anaphylatoxin complement component C3a, [RGD, Feb 2006]

Uniprot Description

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a binding

Biological Process: tolerance induction to nonself antigen; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; positive regulation of smooth muscle cell proliferation; regulation of blood pressure; complement receptor mediated signaling pathway; chemotaxis; positive regulation of cell migration