Mouse complement component 2 ELISA Kit | C2 elisa kit

Mouse Complement C2, C2 ELISA Kit

Cat. Num. AAA913976

• Reactivity: Mouse
• Synonyms: complement component 2; Mouse Complement C2; C2 ELISA Kit; Mouse Complement C2 (C2) ELISA kit; DADB-122G4.1; CO2; DKFZp779M0311; C3/C5 convertase; complement component C2; C2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse C2. No significant cross-reactivity or interference between mouse C2 and analogues was observed.
• Sequence Length: 760

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 18.75 ng/ml-300 ng/ml
• Sensitivity: < 18.75 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for C2 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with C2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for C2. The competitive inhibition reaction is launched between with pre-coated C2 and C2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C2 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 157951694
• NCBI GeneID: 12263
• NCBI Accession #: NP_038512.2
• NCBI GenBank Nucleotide #: NM_013484.2
• UniProt Accession #: P21180; O70350
• Molecular Weight: 84,741 Da
• NCBI Official Full Name: complement C2
• NCBI Official Synonym Full Names: complement component 2 (within H-2S)
• NCBI Official Symbol: C2
• NCBI Protein Information: complement C2; C3/C5 convertase; complement factor C2; MHC complement component C2; classical-complement pathway C3/C5 convertase
• UniProt Protein Name: Complement C2
• Protein Family: Complement C2
• UniProt Gene Name: C2
• UniProt Entry Name: CO2_MOUSE

NCBI Description

This gene encodes component C2 of the classical pathway of the complement system. The encoded protein undergoes proteolytic processing mediated by component C1 resulting in C2a and C2b fragments. C2a fragment, in turn, selectively cleaves components C3 and C5 of the complement system. Mice lacking the encoded protein are found to be more susceptible to bacterial infections. Mutations in the human homolog of this gene are associated with disorders such as systemic lupus erythematosus, Henoch-Schonlein purpura, or polymyositis. [provided by RefSeq, Mar 2015]

Uniprot Description

C2: Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. Defects in C2 are the cause of complement component 2 deficiency (C2D). A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.43

Cellular Component: extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; metal ion binding; serine-type endopeptidase activity; catalytic activity

Biological Process: immune system process; innate immune response; proteolysis; complement activation, classical pathway; complement activation