Human complement component 2 ELISA Kit | C2 elisa kit

Human Complement C2, C2 ELISA Kit

Cat. Num. AAA925742

• Gene Names: C2; CO2; ARMD14
• Reactivity: Human
• Synonyms: complement component 2; Human Complement C2; C2 ELISA Kit; Human Complement C2 (C2) ELISA kit; DADB-122G4.1; CO2; DKFZp779M0311; C3/C5 convertase; complement component C2; C2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human C2. No significant cross-reactivity or interference between human C2 and analogues was observed.
• Sequence Length: 752

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Competitive
• Detection Range: 15.6 ng/ml ng/ml -250 ng/ml.
• Sensitivity: 15.6 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for C2 elisa kit

Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with C2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for C2. The competitive inhibition reaction is launched between with pre-coated C2 and C2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C2 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 14550407
• NCBI GeneID: 717
• NCBI Accession #: NP_000054.2
• NCBI GenBank Nucleotide #: NM_000063.5
• UniProt Accession #: P06681; O19694; Q13904; B4DPF3; B4DV20; E9PFN7
• Molecular Weight: 83,268 Da
• NCBI Official Full Name: complement C2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component 2
• NCBI Official Symbol: C2
• NCBI Official Synonym Symbols: CO2; ARMD14
• NCBI Protein Information: complement C2; C3/C5 convertase; complement component C2
• UniProt Protein Name: Complement C2
• Protein Family: Complement C2
• UniProt Gene Name: C2
• UniProt Entry Name: CO2_HUMAN

NCBI Description

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]

Uniprot Description

C2: Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. Defects in C2 are the cause of complement component 2 deficiency (C2D). A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.43; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase activity; metal ion binding

Biological Process: regulation of complement activation; innate immune response; proteolysis; complement activation, classical pathway; response to nutrient; complement activation

Disease: Macular Degeneration, Age-related, 14; Complement Component 2 Deficiency