Human complement component 1, q subcomponent, B chain ELISA Kit | C1QB elisa kit

Human Complement 1q, C1q ELISA Kit

Cat. Num. AAA704335

• Reactivity: Human
• Synonyms: complement component 1; q subcomponent; B chain; Human Complement 1q; C1q ELISA Kit; beta polypeptide; complement component C1q; complement subcomponent C1q chain B; C1QB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human C1q. No significant cross-reactivity or interference between human C1q and analogues was observed.
• Sequence Length: 253

• Samples: Serum, plasma and tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C1QB elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C1q has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C1q present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C1q is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1q bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 87298828
• NCBI GeneID: 713
• NCBI Accession #: NP_000482.3
• NCBI GenBank Nucleotide #: NM_000491.3
• UniProt Accession #: P02746; Q5T959; Q96H17
• Molecular Weight: 26,722 Da
• NCBI Official Full Name: complement C1q subcomponent subunit B
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, B chain
• NCBI Official Symbol: C1QB
• NCBI Protein Information: complement C1q subcomponent subunit B; complement component C1q, B chain; complement subcomponent C1q chain B; complement component 1, q subcomponent, beta polypeptide
• UniProt Protein Name: Complement C1q subcomponent subunit B
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QB
• UniProt Entry Name: C1QB_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the B-chain polypeptide of human complement subcomponent C1q [provided by RefSeq, Jul 2008]

Uniprot Description

Function: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.