Monkey Complement 1q ELISA Kit | C1q elisa kit

Monkey Complement 1q ELISA Kit

Cat. Num. AAA737260

• Gene Names: C1QTNF5; CTRP5
• Reactivity: Monkey
• Synonyms: Complement 1q; Monkey Complement 1q ELISA Kit; C1q elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This C1q ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C1q. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C1q elisa kit

Principle of the assay: C1q ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C1q. Standards or samples are then added to the microtiter plate wells and C1q if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C1q present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C1q are added to each well to “sandwich” the C1q immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C1q and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1q concentration in each sample is interpolated from this standard curve.

Product Categories/Family for C1q elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 14149712
• NCBI GeneID: 114902
• NCBI Accession #: NP_056460.1
• NCBI GenBank Nucleotide #: NM_015645.4
• Molecular Weight: 25,298 Da
• NCBI Official Full Name: complement C1q tumor necrosis factor-related protein 5
• NCBI Official Synonym Full Names: C1q and tumor necrosis factor related protein 5
• NCBI Official Symbol: C1QTNF5
• NCBI Official Synonym Symbols: CTRP5
• NCBI Protein Information: complement C1q tumor necrosis factor-related protein 5; myonectin; C1q TNF-alpha-related protein 5
• UniProt Protein Name: Complement C1q tumor necrosis factor-related protein 5
• UniProt Gene Name: C1QTNF5
• UniProt Synonym Gene Names: CTRP5; UNQ303/PRO344
• UniProt Entry Name: C1QT5_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that function as components of basement membranes and may play a role in cell adhesion. Mutations in this gene have been associated with late-onset retinal degeneration. The protein may be encoded by either a bicistronic transcript including sequence from the upstream membrane frizzled-related protein gene (MFRP), or by a monocistronic transcript expressed from an internal promoter. [provided by RefSeq, Jun 2013]

Uniprot Description

C1QTNF5: Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD). LORD is an autosomal dominant disorder characterized by onset in the fifth to sixth decade with night blindness and punctate yellow-white deposits in the retinal fundus, progressing to severe central and peripheral degeneration, with choroidal neovascularization and chorioretinal atrophy.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: collagen

Disease: Late-onset Retinal Degeneration