Goat Complement 1q ELISA Kit | C1q elisa kit

Goat Complement 1q ELISA Kit

Cat. Num. AAA737220

• Gene Names: C1QTNF5; CTRP5
• Reactivity: Goat
• Synonyms: Complement 1q; Goat Complement 1q ELISA Kit; C1q elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This C1q ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat C1q. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C1q elisa kit

Principle of the assay: C1q ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C1q antibody and an C1q-HRP conjugate. The assay sample and buffer are incubated together with C1q-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1q concentration since C1q from samples and C1q-HRP conjugate compete for the anti-C1q antibody binding site. Since the number of sites is limited, as more sites are occupied by C1q from the sample, fewer sites are left to bind C1q-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1q concentration in each sample is interpolated from this standard curve.

Product Categories/Family for C1q elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 14149712
• NCBI GeneID: 114902
• NCBI Accession #: NP_056460.1
• NCBI GenBank Nucleotide #: NM_015645.4
• Molecular Weight: 25,298 Da
• NCBI Official Full Name: complement C1q tumor necrosis factor-related protein 5
• NCBI Official Synonym Full Names: C1q and tumor necrosis factor related protein 5
• NCBI Official Symbol: C1QTNF5
• NCBI Official Synonym Symbols: CTRP5
• NCBI Protein Information: complement C1q tumor necrosis factor-related protein 5; myonectin; C1q TNF-alpha-related protein 5
• UniProt Protein Name: Complement C1q tumor necrosis factor-related protein 5
• UniProt Gene Name: C1QTNF5
• UniProt Synonym Gene Names: CTRP5; UNQ303/PRO344
• UniProt Entry Name: C1QT5_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that function as components of basement membranes and may play a role in cell adhesion. Mutations in this gene have been associated with late-onset retinal degeneration. The protein may be encoded by either a bicistronic transcript including sequence from the upstream membrane frizzled-related protein gene (MFRP), or by a monocistronic transcript expressed from an internal promoter. [provided by RefSeq, Jun 2013]

Uniprot Description

C1QTNF5: Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD). LORD is an autosomal dominant disorder characterized by onset in the fifth to sixth decade with night blindness and punctate yellow-white deposits in the retinal fundus, progressing to severe central and peripheral degeneration, with choroidal neovascularization and chorioretinal atrophy.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: collagen

Disease: Late-onset Retinal Degeneration