SDS-PAGE
B7-H5 / Gi24 / VISTA recombinant protein
Recombinant Human B7-H5 / Gi24 / VISTA Protein (His tag)
Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Purity
> 95 % as determined by SDS-PAGE
Synonyms
B7-H5 / Gi24 / VISTA; Recombinant Human B7-H5 / Gi24 / VISTA Protein (His tag); C10orf54; B7-H5 / Gi24 / VISTA recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
311
Application Notes
The recombinant human Gi24 consists of 173 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 38-42 KDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Phe 33
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for B7-H5 / Gi24 / VISTA recombinant protein
Background: C10orf54, also known as GI24, belongs to the immunoglobulin superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, C10orf54 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
Description: A DNA sequence encoding the human Gi24 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the human Gi24 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,908 Da
NCBI Official Full Name
Chromosome 10 open reading frame 54
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
VSIR
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
NCBI Protein Information
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
C10orf54
UniProt Synonym Gene Names
; Sisp-1
Uniprot Description
VISTA: May stimulate MMP14-mediated MMP2 activation
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q22.1
Cellular Component: cell surface; intercellular junction; plasma membrane
Biological Process: cell-cell adhesion
Research Articles on B7-H5 / Gi24 / VISTA
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Product Notes
The B7-H5 / Gi24 / VISTA c10orf54 (Catalog #AAA2545307) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human Gi24 consists of 173 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 38-42 KDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the B7-H5 / Gi24 / VISTA c10orf54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H5 / Gi24 / VISTA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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