Porcine CCAAT/enhancer binding protein beta ELISA Kit | C/EBPbeta elisa kit

Porcine CCAAT/enhancer binding protein beta ELISA Kit

Cat. Num. AAA734235

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Porcine
• Synonyms: CCAAT/enhancer binding protein beta; Porcine CCAAT/enhancer binding protein beta ELISA Kit; C/EBPbeta elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This C/EBP? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine C/EBP?. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C/EBPbeta elisa kit

Principle of the assay: C/EBP? ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C/EBP?. Standards or samples are then added to the microtiter plate wells and C/EBP? if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C/EBP? present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C/EBP? are added to each well to “sandwich” the C/EBP? immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C/EBP? and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C/EBP? concentration in each sample is interpolated from this standard curve.

Product Categories/Family for C/EBPbeta elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.3
• Molecular Weight: 15,938 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: TCF5; PP9092; C/EBP beta; LAP; LIP; TCF-5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; inflammatory response