BUB1A Blocking Peptide | BUB1 blocking peptide

BUB1A Antibody (N-term) Blocking peptide

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Synonyms: BUB1A; BUB1A Antibody (N-term) Blocking peptide; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1; BUB1L; BUB1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1085

• Cellular Location: Nucleus. Chromosome, centromere, kinetochore. Note: Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity)
• Tissue Location: High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for BUB1 blocking peptide

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4757878
• NCBI GeneID: 699
• NCBI Accession #: NP_004327
• NCBI GenBank Nucleotide #: NM_004336.4
• UniProt Accession #: O43683; O43430; O43643; O60626; Q53QE4; E9PC26; F5GXI5
• Molecular Weight: 120,002 Da
• NCBI Official Full Name: mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Protein Family: Checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Protein kinase, Other; Cell cycle regulation; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: cytoplasm; cytosol; kinetochore; membrane; nucleoplasm

Molecular Function: protein binding; protein kinase activity

Biological Process: mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; sister chromatid cohesion