Bone Marrow Stromal Cell Antigen 1 (BST1) Recombinant Protein | BST1 recombinant protein

Recombinant Bone Marrow Stromal Cell Antigen 1 (BST1)

Cat. Num. AAA2010989

• Gene Names: BST1; CD157
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Bone Marrow Stromal Cell Antigen 1 (BST1); Recombinant Bone Marrow Stromal Cell Antigen 1 (BST1); BST1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RNKNCTAI WEAFKVALDK DPCSVLPSDY DLFINLSRHS IPRDKSLFWE NSHLLVNSFA DNTRRFMPLS DVLYGRVADF LSWCRQKNDS GLDYQSCPTS EDCENNPVDS FWKRASIQYS KDSSGVIHVM LNGSEPTGAY PIKGFFADYE IPNLQKEKIT RIEIWVMHEI GGPNVESCGE GSMKVLEKRL KDMGFQYSCI NDYRPVKLLQ CVDHSTHPDC ALKSAAAATQ RKAPSLYTEQ RAGLIIPLFL VLA
• Sequence Length: 318

• Applicable Applications for BST1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg63~Ala313 (Accession # Q10588) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 168229159
• NCBI GeneID: 683
• NCBI Accession #: NP_004325.2
• NCBI GenBank Nucleotide #: NM_004334.2
• UniProt Accession #: Q10588; Q5U0K0; Q96EN3; B2R6A2
• Molecular Weight: 32.3kDa
• NCBI Official Full Name: ADP-ribosyl cyclase 2
• NCBI Official Synonym Full Names: bone marrow stromal cell antigen 1
• NCBI Official Symbol: BST1
• NCBI Official Synonym Symbols: CD157
• NCBI Protein Information: ADP-ribosyl cyclase 2; BST-1; cADPr hydrolase 2; NAD(+) nucleosidase; bone marrow stromal antigen 1; cyclic ADP-ribose hydrolase 2
• UniProt Protein Name: ADP-ribosyl cyclase 2
• Protein Family: GPI inositol-deacylase
• UniProt Gene Name: BST1
• UniProt Synonym Gene Names: BST-1
• UniProt Entry Name: BST1_HUMAN

NCBI Description

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.2.2.6; Hydrolase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: metabolic process; multicellular organismal development; positive regulation of B cell proliferation; humoral immune response