Rabbit anti-Human CD147 Monoclonal Antibody | anti-BSG antibody

Recombinant Anti-CD147 Antibody (FITC), Rabbit Monoclonal

Cat. Num. AAA8101561

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: CD147; Monoclonal Antibody; Recombinant Anti-CD147 Antibody (FITC); Rabbit Monoclonal; CD147/EMMPRIN/Basigin Antibody (FITC); Rabbit MAb; basigin (Ok blood group); Anti-5F7 Antibody; Anti-Basigin Antibody; Anti-CD147 Antibody; Anti-EMMPRIN Antibody; Anti-M6 Antibody; Anti-OK Antibody; Anti-TCSF Antibody; anti-BSG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 125
• Specificity: Human CD147/Basigin/BSG
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-BSG antibody: Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1-10ug/mL
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD147 protein
• Conjugation: FITC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD147/Basigin (rh CD147) and conjugated with FITC under optimum conditions, the unreacted FITC was removed .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of peripheral blood lymphocytes analyzed by flow cytometry. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.)

Flow Cytometry (FC/FACS)

(Profile of anti-CD147 reactivity on HeLa cells, PC-3 cells, A549 cells analyzed by flow cytometry.)

Immunofluorescence (IF)

(Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (5 ug/mL). The image showing membrane staining of HeLa cells. Primary antibody (FITC conjugated) was incubated for 1 hour at 37 degree C.)

Related Product Information for anti-BSG antibody

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

References

Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.

NCBI and Uniprot Product Information

• NCBI GI #: 38372925
• NCBI GeneID: 682
• NCBI Accession #: NP_940991.1
• NCBI GenBank Nucleotide #: NM_198589.2
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: 42,200 Da
• NCBI Official Full Name: basigin isoform 2
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; focal adhesion; membrane; mitochondrion; integral to plasma membrane; melanosome; plasma membrane; sarcolemma; acrosomal membrane; lipid raft

Molecular Function: mannose binding; protein binding

Biological Process: response to peptide hormone stimulus; extracellular matrix organization and biogenesis; response to cAMP; decidualization; odontogenesis of dentine-containing teeth; response to mercury ion; cellular metabolic process; extracellular matrix disassembly; cell surface receptor linked signal transduction; blood coagulation; pyruvate metabolic process; leukocyte migration; embryo implantation

Disease: Blood Group--ok