BRCA1 recombinant protein
Recombinant BRCA1 protein
Purity
The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Synonyms
BRCA1; Recombinant BRCA1 protein; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53; BRCA1 recombinant protein
Host
Baculovirus
Purity/Purification
The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Form/Format
2ug of Recombinant BRCA1 protein and Dilution Buffer AM1 (20mM Tris-Cl (pH8), 20% glycerol, 100mM KCl, 1mM DTT and 0.2mM EDTA).
Protein Species
Human
Tag
His-Tag
Notes
Recombinant BRCA1 is suitable for in vitro function studies including transcription and DNA repair, for protein-protein interaction assays and cell growth assays. 1ng is sufficient for a gelshift assay in a 20ul reaction volume; 50ng is sufficient for reconstituted transcription assays and 100ng is sufficient for protein-protein interaction studies. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Related Product Information for BRCA1 recombinant protein
Short Description: The wild-type BRCA1 protein (1-1863 residues, accession number NM_007294) was expressed in a baculovirus system with an amino terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than>=95% homogeneous and contains no detectable protease, DNase and RNase activity. Breast cancer type 1 susceptibility (BRCA1) protein is a tumor suppressor that is expressed in breast tissue cells and other tissues where it primarily functions to sense and repair DNA damage. BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. BRCA1 associates with other tumor suppressors, DNA repair proteins, and signal transducers in a large complex known as the BRCA1-associated genome surveillance complex (BASC) that functions to identify double-strand DNA breaks and either repair them or destroy cells where DNA damage cannot be repaired. BRCA1 also associates with RNA pol II and histone deacetylase complexes to modulate transcription. In addition to its role in ubiquitination, DNA damage repair and transcriptional regulation, it is known to be required for normal cell cycle progression. Defects in BRCA1 are a cause of susceptibility to familial breast-ovarian cancer type 1 (BROVCA1), a condition associated with familial predisposition to cancer of the breast and ovaries, and pancreatic cancer type 4 (PNCA4). Recombinant BRCA1 is used to study in vitro function, protein-protein interactions and cell growth.
Background: Breast cancer type 1 susceptibility (BRCA1) protein is a tumor suppressor that is expressed in breast tissue cells and other tissues where it primarily functions to sense and repair DNA damage. BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. BRCA1 associates with other tumor suppressors, DNA repair proteins, and signal transducers in a large complex known as the BRCA1-associated genome surveillance complex (BASC) that functions to identify double-strand DNA breaks and either repair them or destroy cells where DNA damage cannot be repaired. BRCA1 also associates with RNA pol II and histone deacetylase complexes to modulate transcription. In addition to its role in ubiquitination, DNA damage repair and transcriptional regulation, it is known to be required for normal cell cycle progression. Defects in BRCA1 are a cause of susceptibility to familial breast-ovarian cancer type 1 (BROVCA1), a condition associated with familial predisposition to cancer of the breast and ovaries, and pancreatic cancer type 4 (PNCA4).
Background: Breast cancer type 1 susceptibility (BRCA1) protein is a tumor suppressor that is expressed in breast tissue cells and other tissues where it primarily functions to sense and repair DNA damage. BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. BRCA1 associates with other tumor suppressors, DNA repair proteins, and signal transducers in a large complex known as the BRCA1-associated genome surveillance complex (BASC) that functions to identify double-strand DNA breaks and either repair them or destroy cells where DNA damage cannot be repaired. BRCA1 also associates with RNA pol II and histone deacetylase complexes to modulate transcription. In addition to its role in ubiquitination, DNA damage repair and transcriptional regulation, it is known to be required for normal cell cycle progression. Defects in BRCA1 are a cause of susceptibility to familial breast-ovarian cancer type 1 (BROVCA1), a condition associated with familial predisposition to cancer of the breast and ovaries, and pancreatic cancer type 4 (PNCA4).
Product Categories/Family for BRCA1 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
205kDa
Protein Family
Similar Products
Product Notes
The BRCA1 (Catalog #AAA388007) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRCA1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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