Brain Natriuretic Peptide Recombinant Protein | BNP recombinant protein

Recombinant Human Brain Natriuretic Peptide (BNP)

Cat. Num. AAA283676

• Applications: Western Blot, ELISA
• Purity: >95 % as determined by SDS-PAGE; Affinity purification
• Synonyms: Brain Natriuretic Peptide; Recombinant Human Brain Natriuretic Peptide (BNP); B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B; BNP recombinant protein

• Host: Yeast
• Purity/Purification: >95 % as determined by SDS-PAGE; Affinity purification
• Form/Format: Lyophilized Powder
• Sequence Length: 1

• Applicable Applications for BNP recombinant protein: Western Blot (WB), ELISA (EIA)
• Application Notes: Western blotting, ELISA, Albumin-bound fluorescence was used in serum of patients with chronic renal failure.
• Species of Origin: Human
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Storage Buffer: Lyophilized from sterile 20mM PB, 240mM NaCl, PH6.0.
• Preparation and Storage: Store it under sterile conditions at -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.Samples are stable for up to twelve months from date of receipt at -88 degree C.

Related Product Information for BNP recombinant protein

Brain natriuretic peptide is 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). BNP is named as such because it was originally identified in extracts of porcine brain, although in humans it is produced mainly in the cardiac ventricles.

BNP is co-secreted along with a 76 amino acid N-terminal fragment (NT-proBNP) which is biologically inactive. BNP binds to and activates the atrial natriuretic factor receptors NPRA, and to a lesser extent NPRB, in a fashion similar to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP, and that of NT-proBNP is even longer, making these peptides better targets than ANP for blood testing.

NCBI and Uniprot Product Information

• NCBI GI #: 4433127
• Molecular Weight: 13.4kDa
• NCBI Official Full Name: brain natriuretic peptide, partial
• Protein Family: Brain natriuretic peptide

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: diuretic hormone activity; hormone activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; negative regulation of cell growth; regulation of vascular permeability; body fluid secretion; regulation of blood vessel size