DNA Topoisomerase-I Native Protein | bTOP1 native protein
Bovine DNA Topoisomerase-I
Applications
Western Blot
Purity
Greater than 70% as determined by SDS-PAGE.
Synonyms
DNA Topoisomerase-I; Bovine DNA Topoisomerase-I; TOP1 Bovine; DNA Topoisomerase-I Bovine; DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70; bTOP1 native protein
Host
Bovine tissues
Purity/Purification
Greater than 70% as determined by SDS-PAGE.
Form/Format
TOP1 is supplied at a concentration of 1mg/1ml in 16mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.
Concentration
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. (varies by lot)
Sequence Length
67
Applicable Applications for bTOP1 native protein
Western Blot (WB)
Application Notes
Western blot with Scleroderma positive patient sera.
Immunological Functions
1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels) and immunodot test with positive/negative sera panels.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for bTOP1 native protein
Description: Bovine DNA Topoisomerase-I purified from bovine tissues by proprietary chromatographic techniques.
Introduction: TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
Introduction: TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The bTOP1 (Catalog #AAA145448) is a Native Protein produced from Bovine tissues and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA Topoisomerase-I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot with Scleroderma positive patient sera. Researchers should empirically determine the suitability of the bTOP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Topoisomerase-I, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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