Chymotrypsin A, Inhibitor
Chymotrypsin A (alpha), Bovine Pancreas, TLCK Treated
Purity
Molecular Biology Grade3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1mM HCl to remove autolysis products and low molecular
Synonyms
Chymotrypsin A; Chymotrypsin A (alpha); Bovine Pancreas; TLCK Treated; inhibitor
Host
Bovine pancreas
Purity/Purification
Molecular Biology Grade
3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1mM HCl to remove autolysis products and low molecular
3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1mM HCl to remove autolysis products and low molecular
Form/Format
Supplied as a dialyzed, lyophilized powder.
CAS Number
9004-07-3
Activity
45 units/mg protein
Trypsin: 0.1%
Trypsin: 0.1%
Quality Control
SDS-PAGE
Unit Definition
1 unit hydrolyzes 1umole of benzoyl-L-tyrosine ethyl ester (BTEE) per minute at 25ºC, pH 7.8. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg using ATEE as a substrate. 1 BTEE unit = 29.5 USP/NF units.
Preparation and Storage
The enzyme is stable for days in solution at pH 3.0. For long term storage as a powder, store at 4 degree C. Protect from moisture.
Related Product Information for Chymotrypsin A, inhibitor
Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues. Chymotrypsinogen A is extracted from pancreatic tissue as a zymogen. Pancreatic extract contains approximately equal amounts of two forms of the zymogen, chymotrypsin A and chymotrypsin B. Chymotrypsin A may be activated to alpha, pi, beta, or gamma chymotrypsin. The enzyme is inhibited by heavy metals, the natural trypsin inhibitors to various degrees, an inhibitor from potato, and organophosphorous compounds.
Product Categories/Family for Chymotrypsin A, inhibitor
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
chymotrypsin (EC 3.4.21.1) - Atlantic cod (fragments)
Protein Family
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Product Notes
The Chymotrypsin A (Catalog #AAA655347) is an Inhibitor produced from Bovine pancreas and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Chymotrypsin A, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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