Bovine Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit | FGF2 elisa kit

Cattle Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

Cat. Num. AAA2700215

• Gene Names: FGF2; BFGF; FGFB; FGF-2; HBGF-2
• Reactivity: Bovine
• Synonyms: Fibroblast Growth Factor 2; Basic (FGF2); Cattle Fibroblast Growth Factor 2; Basic (FGF2) ELISA Kit; B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2; FGF2 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).; No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
• Sequence Length: 288

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 12.35-1000pg/mL
• Sensitivity: < 4.49pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 153285461
• NCBI GeneID: 2247
• NCBI Accession #: NP_001997.5
• NCBI GenBank Nucleotide #: NM_002006.4
• UniProt Accession #: P09038; O00527; P78443; Q16443; Q5PY50; Q7KZ11; Q7KZ72; Q9UC54; Q9UCS5; Q9UCS6; A4LBB8
• Molecular Weight: 21,203 Da
• NCBI Official Full Name: fibroblast growth factor 2 34 kDa isoform
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: FGF2
• NCBI Official Synonym Symbols: BFGF; FGFB; FGF-2; HBGF-2
• NCBI Protein Information: fibroblast growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF2
• UniProt Synonym Gene Names: FGFB; FGF-2; bFGF; HBGF-2

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: extracellular region; extracellular space; nucleus

Molecular Function: 1-phosphatidylinositol-3-kinase activity; chemoattractant activity; cytokine activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; ligand-dependent nuclear receptor transcription coactivator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: activation of MAPK activity; branching involved in ureteric bud morphogenesis; cell migration during sprouting angiogenesis; chemotaxis; embryonic morphogenesis; extracellular matrix organization; fibroblast growth factor receptor signaling pathway; growth factor dependent regulation of skeletal muscle satellite cell proliferation; hyaluronan catabolic process; inositol phosphate biosynthetic process; MAPK cascade; negative regulation of blood vessel endothelial cell migration; negative regulation of fibroblast migration; nervous system development; organ morphogenesis; peptidyl-tyrosine phosphorylation; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cardiac muscle cell proliferation; positive regulation of cell division; positive regulation of cell fate specification; positive regulation of cell proliferation; positive regulation of endothelial cell chemotaxis to fibroblast growth factor; positive regulation of endothelial cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of MAP kinase activity; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of phospholipase C activity; positive regulation of protein kinase B signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; Ras protein signal transduction; regulation of angiogenesis; regulation of endothelial cell chemotaxis to fibroblast growth factor; regulation of receptor activity; release of sequestered calcium ion into cytosol; signal transduction; somatic stem cell maintenance; stem cell proliferation; wound healing