Bovine Beta-Lactoglobulin (bLg) ELISA Kit | bLg elisa kit
Bovine Beta-Lactoglobulin (bLg) ELISA Kit
Gene Names
PAEP; BLG; LGB
Reactivity
Bovine
Synonyms
Beta-Lactoglobulin (bLg); Bovine Beta-Lactoglobulin (bLg) ELISA Kit; LGB; B-LG; bLg elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of bLg. No significant cross-reactivity or interference between bLg and analogues was observed.
Sequence Length
178
Samples
Serum, plasma, tissue homogenates, cell lysates, milk, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.067ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level bLg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level bLg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for bLg elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of bLg in bovine serum, plasma, tissue homogenates, cell lysates, milk, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to bLg. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to bLg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain bLg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of bLg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to bLg. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to bLg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain bLg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of bLg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for bLg elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,883 Da
NCBI Official Full Name
beta-lactoglobulin
NCBI Official Symbol
PAEP
NCBI Official Synonym Symbols
BLG; LGB
NCBI Protein Information
beta-lactoglobulin
UniProt Protein Name
Beta-lactoglobulin
UniProt Gene Name
LGB
UniProt Synonym Gene Names
Beta-LG
UniProt Entry Name
LACB_BOVIN
Uniprot Description
LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted; Inhibitor
