Rabbit anti-Human CDK8 Polyclonal Antibody | anti-CDK8 antibody

CDK8, NT (CDK8, Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35) (AP)

Cat. Num. AAA6284512

• Gene Names: CDK8; K35
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDK8; Polyclonal Antibody; NT (CDK8; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35) (AP); Protein kinase K35; anti-CDK8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CDK8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human CDK8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDK8 antibody (N-term) in Jurkat cell line lysates (35ug/lane). CDK8 (arrow) was detected using the purified Pab.)

Related Product Information for anti-CDK8 antibody

CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Product Categories/Family for anti-CDK8 antibody

Antibodies; Protein Kinases

References

Akoulitchev, S., et al., Nature 407(6800):102-106 (2000). Di Pietro, C., et al., Somat. Cell Mol. Genet. 25(3):185-189 (1999). Rickert, P., et al., Oncogene 18(4):1093-1102 (1999). Tassan, J.P., et al., Proc. Natl. Acad. Sci. U.S.A. 92(19):8871-8875 (1995). Schultz, S.J., et al., Cell Growth Differ. 4(10):821-830 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4502745
• NCBI GeneID: 1024
• NCBI Accession #: NP_001251.1
• NCBI GenBank Nucleotide #: NM_001260.1
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5
• Molecular Weight: 53,284 Da
• NCBI Official Full Name: cyclin-dependent kinase 8
• NCBI Official Synonym Full Names: cyclin-dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35
• NCBI Protein Information: cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK8
• UniProt Entry Name: CDK8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK8: a protein kinase of the CDK family. Along with cyclin C forms part of the RNA polymerase II holoenzyme complex. Phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Regulates transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Protein type: Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.23; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; CMGC group; CDK family; CDK8 subfamily

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; Srb-mediator complex; nucleus

Molecular Function: RNA polymerase subunit kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; protein amino acid phosphorylation