Bovine Interleukin 4 (IL4) CLIA Kit | IL4 clia kit

Bovine Interleukin 4 (IL4) CLIA Kit

Cat. Num. AAA2708590

• Gene Names: IL4; IL-4; BSF-1
• Reactivity: Bovine
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Interleukin 4 (IL4); Bovine Interleukin 4 (IL4) CLIA Kit; Interleukin 4; BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra; IL4 clia kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4).; No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
• Sequence Length: 135

• Applicable Applications for IL4 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 1.05pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 4 (IL4) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 27806871
• NCBI GeneID: 280824
• NCBI Accession #: NP_776346.1
• NCBI GenBank Nucleotide #: NP_776346.1
• UniProt Accession #: P30367
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: IL-4; BSF-1
• NCBI Protein Information: interleukin-4
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_BOVIN

Uniprot Description

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.