Bovine Amphiregulin (AREG) CLIA Kit | AREG clia kit

Bovine Amphiregulin (AREG) CLIA Kit

Cat. Num. AAA2708440

• Reactivity: Bovine
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Amphiregulin (AREG); Bovine Amphiregulin (AREG) CLIA Kit; Amphiregulin; AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor; AREG clia kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Amphiregulin (AREG).; No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.
• Sequence Length: 247

• Applicable Applications for AREG clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 12.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 555958396
• NCBI GeneID: 102270841
• NCBI Accession #: XP_005891318.1
• NCBI GenBank Nucleotide #: XP_005891318.1
• UniProt Accession #: L8IUA9
• NCBI Official Full Name: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Protein Information: amphiregulin
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: M91_08174

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; response to hydrogen peroxide; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; positive regulation of DNA replication; neurite development; positive regulation of phosphorylation