Typical Testing Data/Standard Curve (for reference only)
Free Triiodothyronine (fT3) ELISA Kit | fT3 elisa kit
Free Triiodothyronine (fT3) ELISA Kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine
Synonyms
Free Triiodothyronine (fT3); Free Triiodothyronine (fT3) ELISA Kit; fT3 elisa kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine
Specificity
fT3 ELISA Kit has high sensitivity and excellent specificity for detection of fT3. No significant cross-reactivity or interference between fT3 and analogues was observed.
Assay Type
Competitive
Sensitivity
<2.0 pmol/L
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?15%.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?15%.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for fT3 elisa kit
Principle of the Assay: fT3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT3. A competitive inhibition reaction is launched between fT3 (Standards or samples) and Biotin-conjugated fT3 with the pre-coated antibody specific for fT3. The more amount of fT3 in samples, the less antibody bound by Biotin-conjugated fT3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT3 in the sample. The color development is stopped and the intensity of the color is measured.!!Background/Introduction: Over 99% of Triiodothyronine (T3) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T3 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T3 level changes so that the free T3 concentration remains constant. Thus, measurements of free T3 concentrations correlate more reliably with clinical status than total T3 levels. The increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains basically unchanged.
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Product Notes
The Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine fT3 (Catalog #AAA9711532) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711532 ELISA Kit recognizes Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine fT3. It is sometimes possible for the material contained within the vial of "Free Triiodothyronine (fT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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