Protein A, S. aureus, Cowan Strain Reagent
Protein A, S. aureus, Cowan Strain (Biotin)
Reactivity
Human IgG: 100%Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50%
Rat IgG: 25%
Chicken and Hamster IgG: 1%
Synonyms
Protein A; S. aureus; Cowan Strain; Cowan Strain (Biotin); Cowan Strain reagent
Reactivity
Human IgG: 100%
Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50%
Rat IgG: 25%
Chicken and Hamster IgG: 1%
Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50%
Rat IgG: 25%
Chicken and Hamster IgG: 1%
Form/Format
Supplied as a powder in 10mM HEPES, pH 8, 0.15M sodium chloride. Labeled with Biotin. Reconstitute in 500ul of sterile ddH2O, 0.1% BSA.
Concentration
~2mg/ml (after reconstitution) (varies by lot)
Protein A Biotinlylated
99%
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Protein A, S. aureus, Cowan Strain reagent
Protein A, isolated from a special strain of Staphylococcus aureus, is a 41,000D protein capable of binding to the Fc portion of immunoglobulin. This interaction is not species specific, as Protein A has been shown to bind IgG from a variety of species. The binding was thought to be restricted to IgG, but subsequent studies have demonstrated that Protein A can interact with IgA, IgM and subclasses of IgG from different species. Biotinylated Protein A is a useful reagent for detecting or localizing immunoglobulins on cell surfaces or in tissue sections. Used with the appropriate Avidin D conjugate, immunoglobulins can be studied at the fluorescent or electron microscopic level. Biotinylated Protein A can also be employed for the detection of positive clones in monoclonal antibody production by using Avidin D conjugated with peroxidase or other marker enzymes. Used in many systems as a substitute for a biotinylated second antibody. Because Biotinylated Protein A may produce a high background by binding to endogenous immunoglobulin in the tissue, or it may not bind to the species or class of pri-mary antibody employed, we recommend using biotinylated anti-immunoglobulins for routine immunohistochemical staining.
Solid-phase assay of immunoglobulin reactivity normalized to rabbit IgG.
Solid-phase assay of immunoglobulin reactivity normalized to rabbit IgG.
Product Categories/Family for Protein A, S. aureus, Cowan Strain reagent
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Product Notes
The Protein A, S. aureus, Cowan Strain (Catalog #AAA635420) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. The Protein A, S. aureus, Cowan Strain (Biotin) reacts with Human IgG: 100% Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50% Rat IgG: 25% Chicken and Hamster IgG: 1% and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Protein A, S. aureus, Cowan Strain, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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