Western Blot (WB)
(Western blot analysis on Jurkat cell lysates using Phospho-FER(Tyr402) Ab.The lane on the left was treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse FER (Phospho-Tyr402) Antibody | anti-FER antibody
Rabbit FER (Phospho-Tyr402) Antibody
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis on Jurkat cell lysates using Phospho-FER(Tyr402) Ab.The lane on the left was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on Jurkat cell lysates using Phospho-FER(Tyr402) Ab.The lane on the left was treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(At 1/200 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)
Immunohistochemistry (IHC)
(At 1/200 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)
Immunofluorescence/Immunocytochemistry (IF/ICC)
(Staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary Ab. )
Immunofluorescence/Immunocytochemistry (IF/ICC)
(Staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary Ab. )
Subcellular Location: Cytoplasm. Nucleus. Associated with the chromatin.
Tissue Specificity: Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.
Similarity: The coiled coil domains mediate homooligomerization and are required for location at microtubules.The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes. Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
NCBI and Uniprot Product Information
NCBI Description
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq, Jul 2008]
Uniprot Description
Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.
Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; TK group; Fer family
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; cytoplasm; nuclear chromatin; cell cortex; cytosol; nucleus; cell junction; actin cytoskeleton
Molecular Function: protein binding; cadherin binding; gamma-catenin binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein kinase binding; protein phosphatase 1 binding; Rab GTPase binding; ATP binding; epidermal growth factor receptor binding
Biological Process: regulation of mast cell degranulation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; negative regulation of mast cell activation during immune response; chemotaxis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of cell proliferation; cell adhesion; cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; regulation of epidermal growth factor receptor signaling pathway; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; positive regulation of cell migration
Research Articles on FER
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Product Notes
The FER fer (Catalog #AAA002274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit FER (Phospho-Tyr402) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FER (Phospho-Tyr402) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the FER fer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FER (Phospho-Tyr402), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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