Rabbit anti-Human, Mouse FER (Phospho-Tyr402) Antibody | anti-FER antibody

Rabbit FER (Phospho-Tyr402) Antibody

Cat. Num. AAA2274

• Gene Names: FER; FerT; TYK3; PPP1R74
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: FER (Phospho-Tyr402); Rabbit FER (Phospho-Tyr402) Antibody; anti-FER antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Isotype: IgG
• Specificity: Phospho-FER(Tyr402) Ab detects endogenous levels of FER only when phosphorylated at Tyrosine 402.
• Purity/Purification: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-FER antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 ; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human FER around the phosphorylation site of Tyr402.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on Jurkat cell lysates using Phospho-FER(Tyr402) Ab.The lane on the left was treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(At 1/200 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunofluorescence/Immunocytochemistry (IF/ICC)

(Staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary Ab. )

Related Product Information for anti-FER antibody

Description: Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.

Subcellular Location: Cytoplasm. Nucleus. Associated with the chromatin.

Tissue Specificity: Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.

Similarity: The coiled coil domains mediate homooligomerization and are required for location at microtubules.The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes. Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 119964721
• NCBI GeneID: 2241
• NCBI Accession #: NP_005237.2
• NCBI GenBank Nucleotide #: NM_005246.2
• UniProt Accession #: P16591; B2RCR4; B4DSQ2; H2FLB8
• Molecular Weight: 85kDa
• NCBI Official Full Name: tyrosine-protein kinase Fer
• NCBI Official Synonym Full Names: fer (fps/fes related) tyrosine kinase
• NCBI Official Symbol: FER
• NCBI Official Synonym Symbols: FerT; TYK3; PPP1R74
• NCBI Protein Information: tyrosine-protein kinase Fer; p94-Fer; tyrosine kinase 3; phosphoprotein NCP94; proto-oncogene c-Fer; feline encephalitis virus-related kinase FER; protein phosphatase 1, regulatory subunit 74; fujinami poultry sarcoma/Feline sarcoma-related protein Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferric-chelate reductase
• UniProt Gene Name: FER
• UniProt Synonym Gene Names: TYK3
• UniProt Entry Name: FER_HUMAN

NCBI Description

Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; TK group; Fer family

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; cytoplasm; nuclear chromatin; cell cortex; cytosol; nucleus; cell junction; actin cytoskeleton

Molecular Function: protein binding; cadherin binding; gamma-catenin binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein kinase binding; protein phosphatase 1 binding; Rab GTPase binding; ATP binding; epidermal growth factor receptor binding

Biological Process: regulation of mast cell degranulation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; negative regulation of mast cell activation during immune response; chemotaxis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of cell proliferation; cell adhesion; cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; regulation of epidermal growth factor receptor signaling pathway; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; positive regulation of cell migration