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SDS-Page

Blvrb recombinant protein

Blvrb, 1-206aa, Mouse, His-tag, E Coli

Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Blvrb; 1-206aa; Mouse; His-tag; E Coli; Biliverdin reductase B (flavin reductase (NADPH)); flavin reductase (NADPH) isoform 1; Blvrb recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTVKKIA IFGATGRTGL TTLAQAVQAG YEVTVLVRDS SRLPSEGPQP AHVVVGDVRQ AADVDKTVAG QEAVIVLLGT GNDLSPTTVM SEGTRNIVTA MKAHGVDKVV ACTSAFLLWD PTKVPPRLQD VTDDHIRMHK ILQESGLKYV AVMPPHIGDQ PLTGAYTVTL DGRGPSRVIS KHDLGHFMLR CLTTNEYDGH TTYPSHQYD
Sequence Length
206
Applicable Applications for Blvrb recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for Blvrb recombinant protein
Blvrb, also known as flavin reductase (NADPH) isoform 1, is an enzyme (EC 1.3.1.24) that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. Blvrb is found that major erythrocytic heme catabolic pathway in humans and most mammalian species. Biliverdin reductase is abundantly expressed in kidney, spleen, liver and brain as well as at lower levels in the thymus and minimal levels being detected in testis. Recombinant mouse Blvrb protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Blvrb recombinant protein
References
Baranano DE., et al. (2002). Proc Natl Acad Sci USA. 99 (25):16093-8. Saito F., et al. (1995) Cytogenet Cell Genet. 71 (2):179-81.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24.6 kDa (229aa) confirmed by MALDI-TOF
NCBI Official Full Name
flavin reductase (NADPH) isoform 1
NCBI Official Synonym Full Names
biliverdin reductase B (flavin reductase (NADPH))
NCBI Official Symbol
Blvrb
NCBI Protein Information
flavin reductase (NADPH)
UniProt Protein Name
Flavin reductase (NADPH)
UniProt Gene Name
Blvrb
UniProt Synonym Gene Names
BVR-B; FLR

Uniprot Description

Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe3+ iron to Fe2+ in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

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Product Notes

The Blvrb blvrb (Catalog #AAA206813) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Blvrb can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Blvrb blvrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMTVKKIA IFGATGRTGL TTLAQAVQAG YEVTVLVRDS SRLPSEGPQP AHVVVGDVRQ AADVDKTVAG QEAVIVLLGT GNDLSPTTVM SEGTRNIVTA MKAHGVDKVV ACTSAFLLWD PTKVPPRLQD VTDDHIRMHK ILQESGLKYV AVMPPHIGDQ PLTGAYTVTL DGRGPSRVIS KHDLGHFMLR CLTTNEYDGH TTYPSHQYD. It is sometimes possible for the material contained within the vial of "Blvrb, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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