WWP1 blocking peptide

WWP1 Blocking Peptide

Cat. Num. AAA9621211

• Gene Names: WWP1; AIP5; Tiul1; hSDRP1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: WWP1; WWP1 Blocking Peptide; AIP 5; AIP5; Atropin-1-interacting protein 5; hSDRP1; Nedd 4 like ubiquitin protein ligase; nedd4 like; NEDD4-like E3 ubiquitin-protein ligase WWP1; TGIF interacting ubiquitin ligase 1; Tiul1; WW domain containing E3 ubiquitin protein ligase 1; WW domain-containing protein 1; WWP 1; WWP1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for WWP1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for WWP1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 13654239
• NCBI GeneID: 11059
• NCBI Accession #: NP_008944.1
• NCBI GenBank Nucleotide #: NM_007013.3
• UniProt Accession #: Q9H0M0; O00307; Q5YLC1; Q96BP4
• Molecular Weight: – Da
• NCBI Official Full Name: NEDD4-like E3 ubiquitin-protein ligase WWP1
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 1
• NCBI Official Symbol: WWP1
• NCBI Official Synonym Symbols: AIP5; Tiul1; hSDRP1
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP1
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP1
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP1
• UniProt Synonym Gene Names: AIP5; Tiul1

NCBI Description

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF.