WIPI1 blocking peptide

WIPI1 Antibody (Center) Blocking Peptide

• Gene Names: WIPI1; ATG18; ATG18A; WIPI49
• Synonyms: WIPI1; WIPI1 Antibody (Center) Blocking Peptide; WD repeat domain phosphoinositide-interacting protein 1; WIPI-1; Atg18 protein homolog; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WIPI 49 kDa; WIPI49; WIPI1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 446

• Cellular Location: Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle Preautophagosomal structure membrane; Peripheral membrane protein Cytoplasm, cytoskeleton. Note: Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules
• Tissue Location: Ubiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells Up-regulated in a variety of tumor tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for WIPI1 blocking peptide

Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation- induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 146291100
• NCBI GeneID: 55062
• UniProt Accession #: Q5MNZ9; Q8IXM5; Q9NWF8
• Molecular Weight: 48,202 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 1
• NCBI Official Synonym Full Names: WD repeat domain, phosphoinositide interacting 1
• NCBI Official Symbol: WIPI1
• NCBI Official Synonym Symbols: ATG18; ATG18A; WIPI49
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 1
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 1
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WIPI1
• UniProt Synonym Gene Names: WIPI49; WIPI-1; WIPI 49 kDa
• UniProt Entry Name: WIPI1_HUMAN

NCBI Description

This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

ATG18: May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. Probably interacts with androgen (AR) and the estrogen receptor (ER). Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: cytoplasm; cytosol; endosome membrane; extrinsic to membrane; Golgi membrane; pre-autophagosomal structure membrane; trans-Golgi network

Molecular Function: androgen receptor binding; estrogen receptor binding; phosphatidylinositol 3-phosphate binding; receptor binding

Biological Process: autophagic vacuole formation; autophagy; macroautophagy; mitochondrion degradation; protein amino acid lipidation; vesicle targeting, trans-Golgi to endosome