WBP7 blocking peptide

WBP7 Blocking Peptide

Cat. Num. AAA9627907

• Gene Names: KMT2B; HRX2; MLL2; MLL4; TRX2; WBP7; MLL1B; WBP-7
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: WBP7; WBP7 Blocking Peptide; Histone-lysine N-methyltransferase 2B; HRX2; KIAA0304 gene product; KMT2B; KMT2B_HUMAN; Likely ortholog of mouse WW domain binding protein 7; Lysine N-methyltransferase 2B; mixed lineage leukemia 4; Mixed lineage leukemia gene homolog 2 protein; MLL2; myeloid lymphoid leukemia 4; Myeloid lymphoid or mixed lineage leukemia protein 4; Myeloid/lymphoid or mixed-lineage leukemia protein 4; Trithorax homolog 2; trithorax homologue 2; TRX2; WBP-7; WW domain-binding protein 7; WBP7 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for WBP7 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for WBP7 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 7662046
• NCBI GeneID: 9757
• NCBI Accession #: NP_055542.1
• NCBI GenBank Nucleotide #: NM_014727.2
• UniProt Accession #: Q9UMN6; O15022; O95836; Q96GP2; Q96IP3; Q9UK25; Q9Y668; Q9Y669
• NCBI Official Full Name: histone-lysine N-methyltransferase 2B
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2B
• NCBI Official Symbol: KMT2B
• NCBI Official Synonym Symbols: HRX2; MLL2; MLL4; TRX2; WBP7; MLL1B; WBP-7
• NCBI Protein Information: histone-lysine N-methyltransferase 2B; trithorax homologue 2; WW domain binding protein 7; mixed lineage leukemia gene homolog 2; histone-lysine N-methyltransferase MLL4; myeloid/lymphoid or mixed-lineage leukemia protein 4; myeloid/lymphoid or mixed-line
• UniProt Protein Name: Histone-lysine N-methyltransferase 2B
• UniProt Gene Name: KMT2B
• UniProt Synonym Gene Names: HRX2; KIAA0304; MLL2; MLL4; TRX2; WBP7; Lysine N-methyltransferase 2B; WBP-7
• UniProt Entry Name: KMT2B_HUMAN

NCBI Description

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

MLL2: may be a transcriptional regulator. Contains 3 A.T hook DNA-binding domains. Two alternatively spliced isoforms have been described.

Protein type: Methyltransferase; EC 2.1.1.43; Transcription factor; Methyltransferase, protein lysine; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription factor activity

Biological Process: ovulation; establishment and/or maintenance of chromatin architecture; ovarian follicle development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone H3-K4 methylation; oocyte differentiation; regulation of histone H3-K4 methylation; gene silencing