VASP blocking peptide

VASP Antibody (C-term) Blocking peptide

• Synonyms: VASP; VASP Antibody (C-term) Blocking peptide; Vasodilator-stimulated phosphoprotein; VASP blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of VASP. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 380

• Cellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note: Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
• Tissue Location: Highly expressed in platelets.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VASP blocking peptide

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

NCBI and Uniprot Product Information

• NCBI GI #: 1718079
• NCBI GeneID: 7408
• UniProt Accession #: P50552; Q6PIZ1; Q93035; B2RBT9
• Molecular Weight: 39,830 Da
• NCBI Official Full Name: Vasodilator-stimulated phosphoprotein
• NCBI Official Synonym Full Names: vasodilator-stimulated phosphoprotein
• NCBI Official Symbol: VASP
• NCBI Protein Information: vasodilator-stimulated phosphoprotein
• UniProt Protein Name: Vasodilator-stimulated phosphoprotein
• Protein Family: Vasodilator-stimulated phosphoprotein
• UniProt Gene Name: VASP
• UniProt Synonym Gene Names: VASP
• UniProt Entry Name: VASP_HUMAN

NCBI Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Uniprot Description

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; focal adhesion; plasma membrane

Molecular Function: profilin binding; protein binding

Biological Process: axon guidance; positive regulation of actin filament polymerization