Rabbit VASP Polyclonal Antibody | anti-VASP antibody

VASP (Phospho-Ser157) Antibody

Cat. Num. AAA9410867

• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: VASP; Polyclonal Antibody; VASP (Phospho-Ser157) Antibody; anti-VASP antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of VASP only when phosphorylated at serine 157.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-VASP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 157 (R-V-S(p)-N-A) derived from Human VASP.
• Modification: Phospho-Ser157
• Target Name: VASP
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from C6 cells untreated or treated with PMA using VASP(Phospho-Ser157) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser157) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-VASP antibody

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Product Categories/Family for anti-VASP antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507869
• NCBI GeneID: 7408
• NCBI Accession #: NP_003361.1
• NCBI GenBank Nucleotide #: NM_003370.3
• UniProt Accession #: P50552; Q6PIZ1; Q93035; B2RBT9
• Molecular Weight: 39,830 Da
• NCBI Official Full Name: vasodilator-stimulated phosphoprotein
• NCBI Official Synonym Full Names: vasodilator-stimulated phosphoprotein
• NCBI Official Symbol: VASP
• NCBI Protein Information: vasodilator-stimulated phosphoprotein
• UniProt Protein Name: Vasodilator-stimulated phosphoprotein
• Protein Family: Vasodilator-stimulated phosphoprotein
• UniProt Gene Name: VASP
• UniProt Synonym Gene Names: VASP
• UniProt Entry Name: VASP_HUMAN

NCBI Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Uniprot Description

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding

Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization