VAMP7 blocking peptide

VAMP7 Antibody (Center) Blocking peptide

• Gene Names: VAMP7; SYBL1; TIVAMP; VAMP-7; TI-VAMP
• Synonyms: VAMP7; VAMP7 Antibody (Center) Blocking peptide; Vesicle-associated membrane protein 7; VAMP-7; Synaptobrevin-like protein 1; Tetanus-insensitive VAMP; Ti-VAMP; SYBL1; VAMP7 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of VAMP7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 220

• Cellular Location: Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type IV membrane protein Cell junction, synapse, synaptosome. Note: In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity).
• Tissue Location: Detected in all tissues tested.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VAMP7 blocking peptide

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.

NCBI and Uniprot Product Information

• NCBI GI #: 1723133
• NCBI GeneID: 6845
• UniProt Accession #: P51809; Q53GY7; Q7Z409; Q9H4A7
• Molecular Weight: 20,121 Da
• NCBI Official Full Name: Vesicle-associated membrane protein 7
• NCBI Official Synonym Full Names: vesicle associated membrane protein 7
• NCBI Official Symbol: VAMP7
• NCBI Official Synonym Symbols: SYBL1; TIVAMP; VAMP-7; TI-VAMP
• NCBI Protein Information: vesicle-associated membrane protein 7
• UniProt Protein Name: Vesicle-associated membrane protein 7
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP7
• UniProt Synonym Gene Names: SYBL1; VAMP-7; Ti-VAMP
• UniProt Entry Name: VAMP7_HUMAN

NCBI Description

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

VAMP7: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28 and Yq12

Cellular Component: cytoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle; lamellipodium; late endosome membrane; lysosomal membrane; membrane; neuron projection; phagocytic vesicle; plasma membrane; pseudopodium; secretory granule; secretory granule membrane; SNARE complex; trans-Golgi network

Molecular Function: protein binding; SNAP receptor activity; SNARE binding

Biological Process: calcium ion-dependent exocytosis; endocytosis; endosome to lysosome transport; eosinophil degranulation; ER to Golgi vesicle-mediated transport; natural killer cell degranulation; neutrophil degranulation; phagocytosis, engulfment; post-Golgi vesicle-mediated transport; vesicle fusion; vesicle-mediated transport