USP10 blocking peptide

USP10 Antibody (Center) Blocking Peptide

• Gene Names: USP10; UBPO
• Synonyms: USP10; USP10 Antibody (Center) Blocking Peptide; Ubiquitin carboxyl-terminal hydrolase 10; Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10; KIAA0190; USP10 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 798

• Cellular Location: Cytoplasm. Nucleus. Early endosome. Note: Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for USP10 blocking peptide

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.

NCBI and Uniprot Product Information

• NCBI GI #: 2501458
• NCBI GeneID: 9100
• UniProt Accession #: Q14694; Q9BWG7; Q9NSL7; B2RDJ8; B4DS84
• Molecular Weight: 87,533 Da
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 10
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 10
• NCBI Official Symbol: USP10
• NCBI Official Synonym Symbols: UBPO
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 10
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 10
• UniProt Gene Name: USP10
• UniProt Synonym Gene Names: KIAA0190
• UniProt Entry Name: UBP10_HUMAN

NCBI Description

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

USP10: a carboxyl-terminal ubiquitin-processing protease. Ras-GAP SH3 domain-binding protein (G3BP) appears to inhibit its ability to disassemble ubiquitin chains.

Protein type: Protease; Ubiquitin conjugating system; EC 3.4.19.12; RNA-binding; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: cytoplasm; early endosome; nucleoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; p53 binding; protein binding; ubiquitin-specific protease activity

Biological Process: bypass DNA synthesis; DNA damage response, signal transduction by p53 class mediator; protein deubiquitination; regulation of autophagy