THOC4 blocking peptide

THOC4 Blocking Peptide

Cat. Num. AAA9624835

• Gene Names: ALYREF; ALY; BEF; REF; THOC4; ALY/REF
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: THOC4; THOC4 Blocking Peptide; Ally of AML-1 and LEF-1; Ally of AML1 and LEF1; ALY; ALY/REF; Aly/REF export factor; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; REF; THO complex 4; THO complex subunit 4; Tho4; thoc4; THOC4_HUMAN; Transcriptional coactivator Aly/REF; Transcriptional coactivator; THOC4 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for THOC4 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for THOC4 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 238776833
• NCBI GeneID: 10189
• NCBI Accession #: NP_005773.3
• NCBI GenBank Nucleotide #: NM_005782.3
• UniProt Accession #: Q86V81; O43672
• Molecular Weight: 26,888 Da
• NCBI Official Full Name: THO complex subunit 4
• NCBI Official Synonym Full Names: Aly/REF export factor
• NCBI Official Symbol: ALYREF
• NCBI Official Synonym Symbols: ALY; BEF; REF; THOC4; ALY/REF
• NCBI Protein Information: THO complex subunit 4; tho4; THO complex 4; bZIP enhancing factor; ally of AML-1 and LEF-1; bZIP-enhancing factor BEF; transcriptional coactivator Aly/REF
• UniProt Protein Name: THO complex subunit 4
• UniProt Gene Name: ALYREF
• UniProt Synonym Gene Names: ALY; BEF; THOC4; Tho4
• UniProt Entry Name: THOC4_HUMAN

NCBI Description

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

THOC4: a chaperone protein that promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains thus promoting transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Nuclear localization, translocates to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Two differentially spliced isoforms have been described.

Protein type: Spliceosome; Transcription, coactivator/corepressor; RNA-binding; Chaperone

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; nucleotide binding; single-stranded DNA binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; positive regulation of RNA elongation; regulation of DNA recombination; RNA splicing; gene expression; intronless viral mRNA export from host nucleus; mRNA 3'-end processing; termination of RNA polymerase II transcription; replication fork processing