TERT blocking peptide

TERT Blocking Peptide (S1125)

• Gene Names: TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
• Synonyms: TERT; TERT Blocking Peptide (S1125); Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2; EST2; TCS1; TRT; TERT blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the S1125 region of human TERT. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 1069

• Reconstitution: Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Cellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus Chromosome, telomere. Cytoplasm. Nucleus, PML body. Note=Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT
• Peptide ID: 80118232
• Tissue Location: Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T-lymphocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TERT blocking peptide

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 301129200
• NCBI GeneID: 7015
• NCBI Accession #: NP_001180305.1
• NCBI GenBank Nucleotide #: NM_001193376.1
• UniProt Accession #: O14746; O14783; Q2XS35; Q8N6C3; Q8NG38; Q8NG46
• Molecular Weight: 88,965 Da
• NCBI Official Full Name: telomerase reverse transcriptase isoform 2
• NCBI Official Synonym Full Names: telomerase reverse transcriptase
• NCBI Official Symbol: TERT
• NCBI Official Synonym Symbols: TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
• NCBI Protein Information: telomerase reverse transcriptase
• UniProt Protein Name: Telomerase reverse transcriptase
• Protein Family: Tertiapin
• UniProt Gene Name: TERT
• UniProt Synonym Gene Names: EST2; TCS1; TRT; TP2

NCBI Description

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.

Protein type: DNA-binding; EC 2.7.7.49; Nucleolus; Transferase

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: chromosome, telomeric region; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus; PML body; telomerase holoenzyme complex

Molecular Function: DNA binding; nucleotidyltransferase activity; protein binding; protein homodimerization activity; RNA binding; RNA-directed DNA polymerase activity; RNA-directed RNA polymerase activity; telomerase activity; telomeric DNA binding; telomeric template RNA reverse transcriptase activity; tRNA binding

Biological Process: DNA strand elongation; mitochondrion organization and biogenesis; positive regulation of nitric-oxide synthase activity; positive regulation of protein binding; positive regulation of Wnt receptor signaling pathway; regulation of protein stability; RNA biosynthetic process; RNA interference, production of siRNA; RNA-dependent DNA replication; telomere maintenance; telomere maintenance via telomerase

Disease: Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1; Pulmonary Fibrosis, Idiopathic