SPPL2B blocking peptide

SPPL2B Blocking Peptide (Center)

• Gene Names: SPPL2B; IMP4; PSH4; PSL1; IMP-4
• Synonyms: SPPL2B; SPPL2B Blocking Peptide (Center); Signal peptide peptidase-like 2B; SPP-like 2B; SPPL2b; 3423-; Intramembrane protease 4; IMP-4; Presenilin homologous protein 4; PSH4; Presenilin-like protein 1; IMP4; KIAA1532; PSL1; SPPL2B blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 196-209 of HUMAN SPPL2B
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 592

• Cellular Location: Golgi apparatus membrane; Multi- pass membrane protein. Note: targeted through the entire secretory pathway to endosomes/lysosomes
• Tissue Location: Expressed predominantly in adrenal cortex and mammary gland.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SPPL2B blocking peptide

Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity.

NCBI and Uniprot Product Information

• NCBI GI #: 97537015
• NCBI GeneID: 56928
• UniProt Accession #: Q8TCT7; O60365; Q567S3; Q8IUH9; Q9BUY6; Q9H3M4; Q9NPN2; Q9P1Z6; D6W609
• Molecular Weight: 56,431 Da
• NCBI Official Full Name: Signal peptide peptidase-like 2B
• NCBI Official Synonym Full Names: signal peptide peptidase like 2B
• NCBI Official Symbol: SPPL2B
• NCBI Official Synonym Symbols: IMP4; PSH4; PSL1; IMP-4
• NCBI Protein Information: signal peptide peptidase-like 2B
• UniProt Protein Name: Signal peptide peptidase-like 2B
• Protein Family: Signal peptide peptidase
• UniProt Gene Name: SPPL2B
• UniProt Synonym Gene Names: PSH4
• UniProt Entry Name: SPP2B_HUMAN

NCBI Description

This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SPPL2B: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Belongs to the peptidase A22B family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.23.-; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: actin cytoskeleton; centrosome; endosome membrane; lysosomal membrane; membrane; nucleoplasm; plasma membrane

Molecular Function: aspartic endopeptidase activity, intramembrane cleaving; protein binding; protein homodimerization activity

Biological Process: membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; membrane protein proteolysis; regulation of immune response; signal peptide processing