SIRT6 blocking peptide

SIRT6 Antibody (C-term) Blocking peptide

• Gene Names: SIRT6; SIR2L6
• Synonyms: SIRT6; SIRT6 Antibody (C-term) Blocking peptide; NAD-dependent protein deacetylase sirtuin-6; 351-; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIR2L6; SIRT6 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of SIRT6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 355

• Cellular Location: Nucleus, nucleoplasm. Note: Predominantly nuclear Associated with telomeric heterochromatin regions
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SIRT6 blocking peptide

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.

NCBI and Uniprot Product Information

• NCBI GI #: 38258612
• NCBI GeneID: 51548
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: 36,065 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: SIRT6
• NCBI Official Synonym Symbols: SIR2L6
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIR6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.31; EC 3.5.1.-; Deacetylase; Transferase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nuclear telomeric heterochromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; zinc ion binding

Biological Process: protein amino acid ADP-ribosylation