SHP-2 blocking peptide

SHP2 Blocking Peptide

Cat. Num. AAA9617519

• Gene Names: PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: SHP-2; SHP2 Blocking Peptide; BPTP3; CFC; JMML; METCDS; MGC14433; NS1; OTTHUMP00000166107; OTTHUMP00000166108; Protein tyrosine phosphatase 2; Protein tyrosine phosphatase 2C; Protein tyrosine phosphatase non receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11_HUMAN; PTP-1D; PTP-2C; PTP1D; PTP2C; PTPN11; SAP2; SH-PTP2; SH-PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHP 2; Shp2; SHPTP2; SHPTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11; SHP-2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SHP-2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SHP-2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 1059433492
• NCBI GeneID: 5781
• NCBI Accession #: NP_001317366.1
• NCBI GenBank Nucleotide #: NM_001330437.1
• UniProt Accession #: Q06124; Q96HD7; A8K1D9
• Molecular Weight: 68436 MW
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: PTPN11
• NCBI Official Synonym Symbols: CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• UniProt Gene Name: PTPN11
• UniProt Synonym Gene Names: PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
• UniProt Entry Name: PTN11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; phospholipase binding; SH3/SH2 adaptor activity; peptide hormone receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; phosphoprotein phosphatase activity; D1 dopamine receptor binding; insulin receptor binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; hormone-mediated signaling; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; reproductive process in a multicellular organism; glucose homeostasis; regulation of protein export from nucleus; negative regulation of insulin secretion; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; inner ear development; negative regulation of cell adhesion mediated by integrin; negative regulation of growth hormone secretion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; brain development; platelet formation; blood coagulation; leukocyte migration

Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1