SET blocking peptide

SET Antibody (N-term) Blocking Peptide

• Gene Names: SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• Synonyms: SET; SET Antibody (N-term) Blocking Peptide; Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase; IGAAD; PHAPII; Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I; SET blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 290

• Cellular Location: Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm. Note: In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium
• Tissue Location: Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SET blocking peptide

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

NCBI and Uniprot Product Information

• NCBI GI #: 46397790
• NCBI GeneID: 6418
• UniProt Accession #: Q01105; Q15541; Q5VXV1; Q5VXV2; Q6FHZ5; A5A5H4; A6NGV1; B4DUE2
• Molecular Weight: 31,272 Da
• NCBI Official Full Name: Protein SET
• NCBI Official Synonym Full Names: SET nuclear proto-oncogene
• NCBI Official Symbol: SET
• NCBI Official Synonym Symbols: 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• NCBI Protein Information: protein SET
• UniProt Protein Name: Protein SET
• Protein Family: Set3 complex
• UniProt Gene Name: SET
• UniProt Synonym Gene Names: IGAAD; I-2PP2A; TAF-I
• UniProt Entry Name: SET_HUMAN

NCBI Description

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SET: a potent inhibitor of protein phosphatase 2A involved in apoptosis, transcription, nucleosome assembly and histone binding. The translocation t(6;9)(q21;q34.1) NUP214/CAN is found in some cases of acute undifferentiated leukemia (AUL). Two alternatively spliced isoforms have been described. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Both isoforms inhibit the acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition.

Protein type: DNA-binding; Inhibitor; DNA replication; Oncoprotein; Nuclear receptor co-regulator; Apoptosis

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; endoplasmic reticulum; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: histone binding; protein binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity

Biological Process: DNA replication; negative regulation of histone acetylation; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; nucleosome disassembly; regulation of mRNA stability