SEPT9 blocking peptide

SEPT9 Antibody (C-term) Blocking peptide

• Gene Names: MAFK; P18; NFE2U
• Synonyms: SEPT9; SEPT9 Antibody (C-term) Blocking peptide; Septin-9; MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1; KIAA0991; MSF; SEPT9 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human MAFK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 156

• Cellular Location: Cytoplasm, cytoskeleton Note: In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria
• Tissue Location: Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SEPT9 blocking peptide

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

NCBI and Uniprot Product Information

• NCBI GI #: 4505075
• NCBI GeneID: 7975
• NCBI Accession #: NP_002351
• NCBI GenBank Nucleotide #: NM_002360.3
• UniProt Accession #: Q9UHD8; A4D214; O60675
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: transcription factor MafK
• NCBI Official Synonym Full Names: MAF bZIP transcription factor K
• NCBI Official Symbol: MAFK
• NCBI Official Synonym Symbols: P18; NFE2U
• NCBI Protein Information: transcription factor MafK
• UniProt Protein Name: Transcription factor MafK
• UniProt Gene Name: MAFK
• UniProt Entry Name: MAFK_HUMAN

NCBI Description

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]

Uniprot Description

MAFK: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: nucleoplasm; nucleus

Biological Process: blood coagulation; regulation of transcription from RNA polymerase II promoter