SCYL2 blocking peptide

SCYL2 Antibody(C-term) Blocking peptide

• Gene Names: SCYL2; CVAK104
• Synonyms: SCYL2; SCYL2 Antibody(C-term) Blocking peptide; SCY1-like protein 2; Coated vesicle-associated kinase of 104 kDa; CVAK104; KIAA1360; SCYL2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human SCYL2 (C-term). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 929

• Cellular Location: Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Note: According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans- Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SCYL2 blocking peptide

Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.

NCBI and Uniprot Product Information

• NCBI GI #: 74762350
• NCBI GeneID: 55681
• UniProt Accession #: Q6P3W7; Q96EF4; Q96ST4; Q9H7V5; Q9NVH3; Q9P2I7; A8KAB5
• Molecular Weight: 103,709 Da
• NCBI Official Full Name: SCY1-like protein 2
• NCBI Official Synonym Full Names: SCY1 like pseudokinase 2
• NCBI Official Symbol: SCYL2
• NCBI Official Synonym Symbols: CVAK104
• NCBI Protein Information: SCY1-like protein 2
• UniProt Protein Name: SCY1-like protein 2
• Protein Family: SCY1-like protein
• UniProt Gene Name: SCYL2
• UniProt Synonym Gene Names: CVAK104; KIAA1360
• UniProt Entry Name: SCYL2_HUMAN

NCBI Description

The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

SCYL2: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. Belongs to the protein kinase superfamily.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; SCY1 family

Chromosomal Location of Human Ortholog: 12q23.1

Molecular Function: protein binding; receptor binding

Biological Process: endosome to lysosome transport; positive regulation of receptor internalization