RPA1 blocking peptide

RPA1 Blocking Peptide

Cat. Num. AAA9620041

• Gene Names: POLR1A; A190; RPA1; RPO14; RPA194; RPO1-4
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: RPA1; RPA1 Blocking Peptide; A190; AFDCIN; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit rpa1; Polr1a; Polymerase (RNA) I polypeptide A 194kDa; RNA polymerase I 194 kDa subunit; RNA polymerase I subunit A; RNA polymerase I subunit A1; RPA1_HUMAN; RPA194; RPO1 4; RPO14; RPA1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for RPA1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RPA1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 103471997
• NCBI GeneID: 25885
• NCBI Accession #: NP_056240.2
• NCBI GenBank Nucleotide #: NM_015425.5
• UniProt Accession #: O95602; Q0VG05; Q9UEH0; Q9UFT9; B7Z7T0; D6W5M0
• Molecular Weight: 194,811 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA1
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide A, 194kDa
• NCBI Official Symbol: POLR1A
• NCBI Official Synonym Symbols: A190; RPA1; RPO14; RPA194; RPO1-4
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A1; RNA polymerase I 194 kDa subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I largest subunit
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA1
• Protein Family: Replication factor
• UniProt Gene Name: POLR1A
• UniProt Synonym Gene Names: RNA polymerase I subunit A1; RPA194
• UniProt Entry Name: RPA1_HUMAN

Uniprot Description

POLR1A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleotide Metabolism - pyrimidine; Transferase; Transcription initiation complex; Nucleotide Metabolism - purine; Nucleolus; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic

Disease: Acrofacial Dysostosis, Cincinnati Type