Rnf2 blocking peptide

Rnf2 Blocking Peptide (Center)

• Gene Names: Rnf2; dinG; Ring1B; AI326319; AI450156; AU019207
• Synonyms: Rnf2; Rnf2 Blocking Peptide (Center); E3 ubiquitin-protein ligase RING2; 632-; RING finger protein 1B; RING1b; RING finger protein 2; DinG; Ring1b; Rnf2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 198-212 of HUMAN Rnf2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 336

• Cellular Location: Nucleus. Chromosome. Note: Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Rnf2 blocking peptide

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Association to the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 62901047
• NCBI GeneID: 19821
• UniProt Accession #: Q9CQJ4; O35699; O35729; Q4FJV5; Q8C1X8
• Molecular Weight: 37,623 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RING2
• NCBI Official Synonym Full Names: ring finger protein 2
• NCBI Official Symbol: Rnf2
• NCBI Official Synonym Symbols: dinG; Ring1B; AI326319; AI450156; AU019207
• NCBI Protein Information: E3 ubiquitin-protein ligase RING2
• UniProt Protein Name: E3 ubiquitin-protein ligase RING2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf2
• UniProt Synonym Gene Names: DinG; Ring1b; RING1b
• UniProt Entry Name: RING2_MOUSE

Uniprot Description

RNF2: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin- protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of a PRC1-like complex. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. Interacts with Interacts with PCGF2, CBX4, CBX6, CBX7 and CBX8. Interacts with CBX2, BMI and PHC2. Interacts with RYBP, HIP2 and TFCP2.

Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-

Cellular Component: euchromatin; heterochromatin; nuclear body; nucleoplasm; nucleus; PcG protein complex; sex chromatin; ubiquitin ligase complex

Molecular Function: chromatin binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: anterior/posterior axis specification; gastrulation with mouth forming second; germ cell development; histone ubiquitination; mitotic cell cycle; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter