Western Blot (WB)
((2.5ug/ml) staining of Zebrafish adult Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Thrb Polyclonal Antibody | anti-thrb antibody
Goat anti-Thrb (zebrafish) Antibody
Gene Names
thrb; trb; NR1A2; TR[b]1; TR-beta; fj24f03; wu:fj24f03
Reactivity
Tested: Zebrafish; Expected from sequence similarity: Zebrafish
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Thrb; Polyclonal Antibody; Goat anti-Thrb (zebrafish) Antibody; thrb; thyroid hormone receptor beta; NR1A2; TR-beta; TR[b]1; fj24f03; trb; wu: fj24f03; thrb antibody; thyroid hormone receptor beta antibody; NR1A2 antibody; TR-beta antibody; TR[b]1 antibody; fj24f03 antibody; trb antibody; wu: fj24f03 antibody; Thrb (zebrafish); anti-thrb antibody
Host
Goat
Reactivity
Tested: Zebrafish; Expected from sequence similarity: Zebrafish
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DIGSAPIVNAPEGNKVD
Sequence Length
386
Applicable Applications for anti-thrb antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 50kDa band observed in Zebrafish adult Heart lysates (calculated MW of 44.3kDa according to NP_571415.1). Recommended concentration: 2-5ug/ml.
Western Blot: Approx 50kDa band observed in Zebrafish adult Heart lysates (calculated MW of 44.3kDa according to NP_571415.1). Recommended concentration: 2-5ug/ml.
Immunogen
Peptide with sequence C-DIGSAPIVNAPEGNKVD, from the internal region of the protein sequence according to NP_571415.1.
Epitope
Internal region
Note
This antibody is not expected to cross-react to Thra.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2.5ug/ml) staining of Zebrafish adult Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2.5ug/ml) staining of Zebrafish adult Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,285 Da
NCBI Official Full Name
thyroid hormone receptor beta
NCBI Official Synonym Full Names
thyroid hormone receptor beta
NCBI Official Symbol
thrb
NCBI Official Synonym Symbols
trb; NR1A2; TR[b]1; TR-beta; fj24f03; wu:fj24f03
NCBI Protein Information
thyroid hormone receptor beta
UniProt Protein Name
Thyroid hormone receptor beta
Protein Family
UniProt Gene Name
thrb
UniProt Synonym Gene Names
nr1a2; trb; TR-beta; TRb; TRbeta1
UniProt Entry Name
THB_DANRE
Uniprot Description
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for the thyroid gland hormone triiodothyronine (T3). Transactivating activity is ligand-dependent, and is repressed in the absence of T3.
Research Articles on thrb
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Product Notes
The thrb thrb (Catalog #AAA420704) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Thrb (zebrafish) Antibody reacts with Tested: Zebrafish; Expected from sequence similarity: Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Thrb can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in Zebrafish adult Heart lysates (calculated MW of 44.3kDa according to NP_571415.1). Recommended concentration: 2-5ug/ml. Researchers should empirically determine the suitability of the thrb thrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DIGSAPIVNA PEGNKVD. It is sometimes possible for the material contained within the vial of "Thrb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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