RALA blocking peptide

RALA Antibody (C-term) Blocking Peptide

• Gene Names: RALA; RAL
• Synonyms: RALA; RALA Antibody (C-term) Blocking Peptide; Ras-related protein Ral-A; RAL; RALA blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 206

• Cellular Location: Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody. Note: Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA colocalizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RALA blocking peptide

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 131834
• NCBI GeneID: 5898
• UniProt Accession #: P11233; A4D1W3
• Molecular Weight: 23,567 Da
• NCBI Official Full Name: Ras-related protein Ral-A
• NCBI Official Synonym Full Names: RALA Ras like proto-oncogene A
• NCBI Official Symbol: RALA
• NCBI Official Synonym Symbols: RAL
• NCBI Protein Information: ras-related protein Ral-A
• UniProt Protein Name: Ras-related protein Ral-A
• Protein Family: Ras-related protein
• UniProt Gene Name: RALA
• UniProt Synonym Gene Names: RAL
• UniProt Entry Name: RALA_HUMAN

NCBI Description

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: cell surface; cleavage furrow; cytoplasmic vesicle membrane; endocytic vesicle; focal adhesion; plasma membrane

Molecular Function: ATPase binding; Edg-2 lysophosphatidic acid receptor binding; GDP binding; GTP binding; myosin binding; protein binding; ubiquitin protein ligase binding

Biological Process: actin cytoskeleton reorganization; chemotaxis; cytokinesis; lipid raft localization; positive regulation of filopodium formation; regulation of exocytosis; signal transduction