Mouse CNPase Monoclonal Antibody

CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase)

Cat. Num. AAA603287

• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: CNPase; Monoclonal Antibody; CNPase (2'; 3'-cyclic Nucleotide 3'-phosphodiesterase); Anti -CNPase (2'; anti-CNPase antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q33
• Specificity: Recognizes humn CNPase, 48 and 46kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc. Species Crossreactivity: bovine, rat, mouse, rabbit, sheep, porcine, canine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.

• Applicable Applications for anti-CNPase antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.; Dilution: Western Blot: 1:1,000 using 10ug of mouse brain lysate as sample. Other suitable samples include myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections; and mixed glial tumors (oligodendrogliomas, etc.); Immunohistochemistry: Fresh frozen and paraffin embedded tissue. Note: Recommend microwave pretreatment, ctirate pH 6.0 of paraffin sections.; Immunocytochemistry: Primary oligodendrocyte cultures.
• Immunogen: Purified human brain CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase) (whloe protein).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Localization of CNPase in rat hippocampus.)

Testing Data

Related Product Information for anti-CNPase antibody

CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. CNPase has been very useful as a marker for these particular cell types. Developmentally, this enzyme appears early in oligodendrocytes, earlier than most other myelin proteins. It continues to be expressed at high levels in oligodendrocytes and Schwann cells of adult animals. In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNPase levels may be severely reduced. The enzyme itself exists in two forms in most species (three in mouse) with molecular masses of 48 and 46kD on SDS-PAGE, referred to as CNP2 and CNP1, respectively.Studies continue to elucidate the physiological role of the enzyme. CNPase I (46kD) and CNPase II (48kD) are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.

Product Categories/Family for anti-CNPase antibody

Antibodies; Abs to Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 380763699
• NCBI Official Full Name: Chain A, Catalytic Domain Of Mouse 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase, Soaked With 2',3'-Cyclic Amp