Raf-B blocking peptide

Raf-B (K439) Peptide

Cat. Num. AAA3004231

• Gene Names: BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
• Applications: Blocking
• Purity: Synthetic peptide Raf-B (K439). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: Raf-B; Raf-B (K439) Peptide; TAF1B; MGC: 9349; RAF1B; RAFI63; SL1; TAFI63; Raf-B blocking peptide

• Specificity: This peptide can be used with studies using MBS3004230 Raf-B (K439) pAb.
• Purity/Purification: Synthetic peptide Raf-B (K439). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 766

• Applicable Applications for Raf-B blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for Raf-B blocking peptide

Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.

NCBI and Uniprot Product Information

• NCBI GI #: 33188459
• NCBI GeneID: 673
• NCBI Accession #: NP_004324.2
• NCBI GenBank Nucleotide #: NP_004324.2
• UniProt Accession #: P15056
• NCBI Official Full Name: serine/threonine-protein kinase B-raf isoform 1
• NCBI Official Synonym Full Names: B-Raf proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: BRAF
• NCBI Official Synonym Symbols: NS7; B-raf; BRAF1; RAFB1; B-RAF1
• NCBI Protein Information: serine/threonine-protein kinase B-raf
• UniProt Protein Name: Serine/threonine-protein kinase B-raf
• UniProt Gene Name: BRAF
• UniProt Synonym Gene Names: BRAF1; RAFB1
• UniProt Entry Name: BRAF_HUMAN

NCBI Description

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Uniprot Description

BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Protein kinase, TKL; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus

Molecular Function: small GTPase binding; identical protein binding; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; positive T cell selection; response to cAMP; fibroblast growth factor receptor signaling pathway; CD4-positive, alpha beta T cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; somatic stem cell maintenance; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Leopard Syndrome 3; Cardiofaciocutaneous Syndrome 1