RAD1 blocking peptide

RAD1 Blocking Peptide

Cat. Num. AAA9622597

• Gene Names: RAD1; REC1; HRAD1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: RAD1; RAD1 Blocking Peptide; Cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein Rad 1 A/B; Cell cycle checkpoint protein RAD1; Checkpoint control protein HRAD1; Checkpoint control protein RAD1; DNA repair exonuclease; DNA repair exonuclease rad1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; DNA repair protein RAD1; EC 3.1.11.2; Exonuclease homolog RAD1; GTP-binding protein RAD; hRAD 1; hRAD1; MGC77779; RAD 1; RAD; RAD1 homolog (S. pombe); RAD1 homolog; Rad1 like DNA damage checkpoint; Rad1 like DNA damage checkpoint protein; S. pombe; homolog of; Rad1-like DNA damage checkpoint protein; RAD1_HUMAN; Ras associated with diabetes; REC 1; REC1; RRAD; RAD1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for RAD1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAD1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4506385
• NCBI GeneID: 5810
• NCBI Accession #: NP_002844.1
• NCBI GenBank Nucleotide #: NM_002853.3
• UniProt Accession #: O60671; O75572; O95304; Q1W161; Q5KSM0; Q5KSM1; Q9UEP1
• Molecular Weight: 7,576 Da
• NCBI Official Full Name: cell cycle checkpoint protein RAD1
• NCBI Official Synonym Full Names: RAD1 checkpoint DNA exonuclease
• NCBI Official Symbol: RAD1
• NCBI Official Synonym Symbols: REC1; HRAD1
• NCBI Protein Information: cell cycle checkpoint protein RAD1; DNA repair exonuclease REC1; DNA repair exonuclease rad1 homolog; RAD1 homolog; cell cycle checkpoint protein Hrad1; checkpoint control protein HRAD1; exonuclease homolog RAD1; rad1-like DNA damage checkpoint protein
• UniProt Protein Name: Cell cycle checkpoint protein RAD1
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD1
• UniProt Synonym Gene Names: REC1; hRAD1
• UniProt Entry Name: RAD1_HUMAN

NCBI Description

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

RAD1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. Belongs to the rad1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA repair, damage; Deoxyribonuclease; EC 3.1.11.2

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; chromosome; nucleus

Molecular Function: protein binding; exodeoxyribonuclease III activity; damaged DNA binding; 3'-5' exonuclease activity

Biological Process: meiotic recombination checkpoint; substantia nigra development; DNA damage checkpoint; DNA repair; DNA catabolic process, exonucleolytic; DNA replication; response to DNA damage stimulus