PSTPIP1 blocking peptide

PSTPIP1 Antibody (N-term) Blocking peptide

• Gene Names: PSTPIP1; H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• Synonyms: PSTPIP1; PSTPIP1 Antibody (N-term) Blocking peptide; Proline-serine-threonine phosphatase-interacting protein 1; PEST phosphatase-interacting protein 1; CD2-binding protein 1; H-PIP; CD2BP1; PSTPIP1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 416

• Cellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, uropodium. Cytoplasm, perinuclear region. Cleavage furrow. Note: During interphase, colocalizes with F- actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity). In monocytes, forms a branched filamentous network in the cytoplasm. In migrating neutrophils, localizes most strongly to the trailing edge of the uropod where it colocalizes with PIP5K1C. In transfected cells, forms relatively straight filaments radiating out from the nucleus. Colocalizes with MEFV, particularly at the branch point of filaments. Filament formation requires an intact tubulin cytoskeleton.
• Tissue Location: Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PSTPIP1 blocking peptide

Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.

NCBI and Uniprot Product Information

• NCBI GI #: 74739557
• NCBI GeneID: 9051
• UniProt Accession #: O43586; O43585; O95657; B5BU74; B5BUK4
• Molecular Weight: 45,354 Da
• NCBI Official Full Name: Proline-serine-threonine phosphatase-interacting protein 1
• NCBI Official Synonym Full Names: proline-serine-threonine phosphatase interacting protein 1
• NCBI Official Symbol: PSTPIP1
• NCBI Official Synonym Symbols: H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• NCBI Protein Information: proline-serine-threonine phosphatase-interacting protein 1
• UniProt Protein Name: Proline-serine-threonine phosphatase-interacting protein 1
• Protein Family: Proline-serine-threonine phosphatase-interacting protein
• UniProt Gene Name: PSTPIP1
• UniProt Synonym Gene Names: CD2BP1; PEST phosphatase-interacting protein 1
• UniProt Entry Name: PPIP1_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response. [provided by RefSeq, Mar 2016]

Uniprot Description

PSTPIP1: an SH3-domain containing protein that may play a role in vesicle formation and transport. Interacts with PTP-PEST to inhibit WASp-driven actin polymerization and synapse formation.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: cytosol; membrane

Molecular Function: protein binding

Biological Process: signal transduction

Disease: Pyogenic Sterile Arthritis, Pyoderma Gangrenosum, And Acne